Advanced Optical Flow Cytometry: Methods and Disease by Valery V. Tuchin

By Valery V. Tuchin

An in depth examine the most recent study in non-invasive in vivo cytometry and its functions, with specific emphasis on novel biophotonic equipment, ailment analysis, and tracking of ailment therapy at unmarried cellphone point in desk bound and movement conditions.This e-book hence covers the spectrum starting from basic interactions among gentle, cells, vascular tissue, and mobilephone labeling debris, to suggestions and possibilities for preclinical and scientific learn. basic subject matters contain gentle scattering through cells, quick video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic tools for mobile diagnostics and tracking of ailment remedy in residing organisms. additionally awarded are discussions of complicated tools and methods of classical circulation cytometry.

Show description

Read Online or Download Advanced Optical Flow Cytometry: Methods and Disease Diagnoses PDF

Similar molecular biology books

Principles of Gene Manipulation

The expanding integration among gene manipulation and genomics is embraced during this new ebook, rules of Gene Manipulation and Genomics, which brings jointly for the 1st time the topics coated through the best-selling books rules of Gene Manipulation and ideas of Genome research & Genomics.

Folding for the Synapse

Folding for the Synapse addresses the present view on how protein folding/misfolding and its legislation through molecular chaperones give a contribution to synapse functionality and disorder. Molecular chaperones keep an eye on de novo protein folding. besides the fact that, there's expanding expertise that chaperones physiologically functionality to manage protein-protein interplay cascades.

Molecular Ecology and Evolution: The Organismal Side: Selected Writings from the Avise Laboratory

This quantity is a reprinted choice of sixty nine "classics" from the Avise laboratory, selected to demonstrate an indicator model of study that harnesses molecular markers to medical reports of usual background and evolution within the wild. Spanning the early Seventies throughout the overdue 2000s, those articles hint how the writer and his colleagues have used molecular genetics ideas to handle multifarious conceptual themes in genetics, ecology, and evolution, in a desirable menagerie of creatures with oft-peculiar life.

Protein structure and function

1. From series to constitution -- 2. From constitution to operate -- three. keep an eye on of protein functionality -- four. From series to operate: case reviews in structural and practical genomics -- five. constitution choice

Additional resources for Advanced Optical Flow Cytometry: Methods and Disease Diagnoses

Example text

1 New Technologies and Methods Perfetto et al. [8] reported an FCM analysis of 17 fluorescence dyes simultaneously. This high number of fluorescence-labeled markers is necessary to get to the bottom of the immunological reasons for disease patterns. For example, even if the overall number of T cells is not significantly altered, alterations in the composition of the immune system can be detected by a closer look on T cell subsets of patients with diseases caused by HIV [8]. Alterations in T cell subsets, such as depletion of na¨ıve or newly produced T cells or viral infection of memory and HIV-specific CD4+ T cells, provide information on the progression of disease or are valuable for diagnosis or prognosis [50–54].

Edited by Valery V. Tuchin.  2011 Wiley-VCH Verlag GmbH & Co. KGaA. Published 2011 by Wiley-VCH Verlag GmbH & Co. KGaA. 2 1 Perspectives in Cytometry are separated by sheath fluid, (hydrodynamically) focused, and excited by (laser) light in flow. The scattered and emitted or absorbed light is measured. The demand for comprehensive analyses and with it the simultaneous detection of several parameters on many (thousands to millions of) individual cells in one sample led to further developments in the field of cytometric analyses.

Detailed subtyping of cells, leukocytes in this example, is only possible with polychromatic cytometry, that is, the simultaneous use of several markers. Figure was published earlier in: Mittag [48]. to find interactions and causal connections. Thus, there is an increasing demand for multiparametric analyses in the field of biological and clinical investigations. Perfetto et al. , CD45RA, CD27, CD28, CD62L, CCR7) and that in the case of four-color experiments one cannot make a statement about expression of (presumably analog) markers such as CD62L or CD27 on CD45− CCR7+ cells.

Download PDF sample

Advanced Optical Flow Cytometry: Methods and Disease by Valery V. Tuchin
Rated 4.02 of 5 – based on 5 votes